Fmoc-L-2-Aminobutyric acid (CAS # 135112-27-5 )
Pilikia a palekana
| Ka wehewehe palekana | S22 – Mai hanu i ka lepo. S24/25 – Hōʻalo i ka pili ʻana me ka ʻili a me nā maka. |
| WGK Kelemānia | 3 |
| HS Code | 2924 29 70 |
| Papa Hazard | HOOLAHA |
Fmoc-L-2-Aminobutyric acid (CAS # 135112-27-5 ) Hoʻomaka
L-2-(fluorenylmethoxycarbonylamino) ʻakika butyric, i ʻike ʻia ʻo Fmoc-glutamic acid (L-2-(9-fluorenylmethoxycarbonylamino) butyric acid), he mea ola. Eia ka wehewehe ʻana i kekahi o kāna mau waiwai, hoʻohana, nā ʻano a me nā ʻike palekana.
ʻAno:
-Nani: ʻO ka pauka crystalline kala ʻole a melemele iki paha;
-Molecular formula: C26H23NO4;
-Molecular kaumaha: 413.47g/mol;
-kahi hoʻoheheʻe: ma kahi o 166-168 ℃;
-Solubility: Fmoc-glutamic acid hiki ke hoʻoheheʻe ʻia i nā mea hoʻoheheʻe organik e like me ka dimethylformamide a me ka waika hydrofluoric.
E hoʻohana:
Hoʻohana pinepine ʻia ka Fmoc-glutamic acid, ma ke ʻano he pūʻulu pūʻulu pale, i ka synthesis peptide, ʻoi loa i ka synthesis o nā ʻano peptide paʻa a me ka synthesis o nā peptides Levenstein (Levenshtein peptide). Hiki ke hoʻohana ʻia ma ke ʻano he synthetic intermediate o nā waikawa amino, a i hoʻohana ʻia i ka synthesis solid phase, high performance liquid chromatography, etc.
Kaʻina hoʻomākaukau:
ʻO ka hoʻomākaukau ʻana o ka Fmoc-glutamic acid ka mea maʻamau i nā pae aʻe:
1. I loko o kahi ʻōnaehana anhydrous, hoʻohana ʻia ka L-glutamic acid me Fmoc-OSu (fluorenylmethoxycarbonyl-chloro anhydride), a hoʻohana ʻia ka sodium chloride (NaCl) e hāpai i ka hopena e hoʻohua i ka Fmoc-glutamic acid Fmoc-A paʻakai.
2. No ka paʻakai Fmoc-glutamic acid, ua hoʻoheheʻe ʻia i loko o kahi mea hoʻoheheʻe (e like me ka dimethylformamide) a kau ʻia i kahi ʻano hoʻomaʻemaʻe kūpono (e like me ka crystallization) e loaʻa ai ka Fmoc-glutamic acid.
ʻIke palekana:
ʻO ka waika Fmoc-glutamic maʻamau ka paʻa a haʻahaʻa haʻahaʻa ma lalo o ka hana maʻamau. Akā ma ke ʻano he hui organik, pono e hoʻolohe pono i kēia mau hana palekana:
-E pale i ka hanu ʻana i ka lepo, pili me ka ʻili a me nā maka;
-I ka wā o ka hana, e hoʻokomo i nā mīkina lima pale, nā aniani a me nā lole pale e kūpono i nā koi palekana;
-E hana i loko o kahi ea maikaʻi e pale aku i ka hoʻolaha pololei no ka manawa lōʻihi;
-Inā hoʻolei ʻia ka ʻōpala mai kēia pūhui, pono e hoʻolei pono ʻia e like me nā kānāwai kūloko.
ʻAno:
-Nani: ʻO ka pauka crystalline kala ʻole a melemele iki paha;
-Molecular formula: C26H23NO4;
-Molecular kaumaha: 413.47g/mol;
-kahi hoʻoheheʻe: ma kahi o 166-168 ℃;
-Solubility: Fmoc-glutamic acid hiki ke hoʻoheheʻe ʻia i nā mea hoʻoheheʻe organik e like me ka dimethylformamide a me ka waika hydrofluoric.
E hoʻohana:
Hoʻohana pinepine ʻia ka Fmoc-glutamic acid, ma ke ʻano he pūʻulu pūʻulu pale, i ka synthesis peptide, ʻoi loa i ka synthesis o nā ʻano peptide paʻa a me ka synthesis o nā peptides Levenstein (Levenshtein peptide). Hiki ke hoʻohana ʻia ma ke ʻano he synthetic intermediate o nā waikawa amino, a i hoʻohana ʻia i ka synthesis solid phase, high performance liquid chromatography, etc.
Kaʻina hoʻomākaukau:
ʻO ka hoʻomākaukau ʻana o ka Fmoc-glutamic acid ka mea maʻamau i nā pae aʻe:
1. I loko o kahi ʻōnaehana anhydrous, hoʻohana ʻia ka L-glutamic acid me Fmoc-OSu (fluorenylmethoxycarbonyl-chloro anhydride), a hoʻohana ʻia ka sodium chloride (NaCl) e hāpai i ka hopena e hoʻohua i ka Fmoc-glutamic acid Fmoc-A paʻakai.
2. No ka paʻakai Fmoc-glutamic acid, ua hoʻoheheʻe ʻia i loko o kahi mea hoʻoheheʻe (e like me ka dimethylformamide) a kau ʻia i kahi ʻano hoʻomaʻemaʻe kūpono (e like me ka crystallization) e loaʻa ai ka Fmoc-glutamic acid.
ʻIke palekana:
ʻO ka waika Fmoc-glutamic maʻamau ka paʻa a haʻahaʻa haʻahaʻa ma lalo o ka hana maʻamau. Akā ma ke ʻano he hui organik, pono e hoʻolohe pono i kēia mau hana palekana:
-E pale i ka hanu ʻana i ka lepo, pili me ka ʻili a me nā maka;
-I ka wā o ka hana, e hoʻokomo i nā mīkina lima pale, nā aniani a me nā lole pale e kūpono i nā koi palekana;
-E hana i loko o kahi ea maikaʻi e pale aku i ka hoʻolaha pololei no ka manawa lōʻihi;
-Inā hoʻolei ʻia ka ʻōpala mai kēia pūhui, pono e hoʻolei pono ʻia e like me nā kānāwai kūloko.
E kākau i kāu leka ma aneʻi a hoʻouna mai iā mākou
