Isopropyl-beta-D-thiogalactopyranoside（CAS#367-93-1）
Pilikia a palekana
| Nā Code Risk | R19 - Hiki ke hana i nā peroxide pahū R40 - Nā hōʻike liʻiliʻi o ka hopena carcinogenic R66 – Hiki ke hoʻomaloʻo a pohā paha ka ʻili i ka hoʻolaha pinepine ʻia R36/37/38 – Hoʻonanea i ka maka, ka ʻōpū hanu a me ka ʻili. |
| Ka wehewehe palekana | S23 – Mai hanu i ka mahu. S24/25 – Hōʻalo i ka pili ʻana me ka ʻili a me nā maka. S36/37 – E hoʻohana i nā lole pale kūpono a me nā mīkina lima. S22 – Mai hanu i ka lepo. S36/37/39 – E hoʻohana i nā lole pale kūpono, nā mīkina lima a me ka pale maka/maka. S27 – Wehe koke i na lole i haumia. S26 – Inā pili me nā maka, e holoi koke me ka wai nui a e ʻimi i ka ʻōlelo aʻo kino. |
| WGK Kelemānia | 3 |
| NUI FLUKA BRAND F | 10 |
| TSCA | ʻAe |
| HS Code | 29389090 |
Hoʻolauna
ʻO ka IPTG kahi mea hoʻoulu hana o β-galactosidase. Ma muli o kēia hiʻohiʻona, ke hoʻololi ʻia ka DNA vector o ka pUC series (a i ʻole DNA vector ʻē aʻe me ka lacZ gene) me ka lacZ deletion cell ma ke ʻano he host, a i ʻole ke hoʻololi ʻia ka vector DNA o M13 phage, inā hoʻohui ʻia ʻo X-gal a me IPTG. i ka pae pā, ma muli o ka α-complementarity o β-galactosidase, hiki ke koho maʻalahi ka gene recombinant e like me ka ʻike ʻana o nā kolone keʻokeʻo (a i ʻole nā pēpē). Eia hou, hiki ke hoʻohana ʻia ma ke ʻano he hōʻike inducer no nā vectors hōʻike me nā mea hoʻolaha e like me lac a tac paha. Hiki ke hoʻoheheʻe ʻia i ka wai, methanol, ethanol, hiki ke hoʻoheheʻe ʻia i ka acetone, chloroform, hiki ʻole ke hoʻoheheʻe ʻia i ka etera. He mea hoʻoulu ia o β-galactosidase a me β-galactosidase. ʻAʻole ia i hydrolyzed e β-galactoside. He hopena substrate ia o ka thiogalactosyltransferase. Hana ʻia: Hoʻoheheʻe ʻia ʻo IPTG i ka wai, a laila sterilized e hoʻomākaukau i kahi hopena mālama (0 · 1M). ʻO ka manaʻo hope loa o ka IPTG ma ka pā hōʻailona he 0 · 2mM.
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