N(alpha)-fmoc-N(epsilon)-(2-chloro-Z)-L-lysine(CAS# 133970-31-7)

ʻO Fmoc-(2-chlorobenzyloxycarbonyl) lysine ka mea hoʻohana maʻamau i hoʻohana ʻia me nā waiwai penei:1. Nānā: ka pauka crystalline keʻokeʻo;
2. kumu mole: C26H24ClNO5;
3. Kaumaha Molecular: 459.92g/mol;
4. Solubility: Hiki ke hoʻoheheʻeʻia i loko o nā mea hoʻoheheʻe organik e like me dimethyl sulfoxide (DMSO), dimethyl formamide (DMF), dichloromethane, a me nā mea'ē aʻe, hikiʻole ke hoʻololi i ka wai;
5. Ka helu heheʻe: e pili ana i 170-175 ° C. ʻO ka hoʻohana mua ʻana o Fmoc-(2-chlorobenzyloxycarbonyl) lysine ma ke ʻano he hui pale a hoʻoulu i ka synthesis o polypeptides. Hiki ke ho'ā 'ia kona pū'ulu carboxyl e hana i ka ester, a laila e hana 'ia ka condensation me ke koena amino acid e synthesize i ke kaulahao polypeptide. Hiki ke wehe maʻalahi ka hui Fmoc ma hope o ka pau ʻana o ka hopena e hōʻike i ka ʻāpana amino i pale ʻia.

ʻO ke ʻano o ka hoʻomākaukau ʻana i ka Fmoc-(2-chlorobenzyloxycarbonyl) lysine e pili ana i kēia mau ʻanuʻu:

1. ka hana ʻana i ka lysine me ka N-hydroxybutyrimide (Pbf) e hoʻolauna i kahi hui pale;
2. ka hoʻopili ʻana i ka lysine-Pbf derivative me ka waiʻona 2-chlorobenzyl e hana i ka lysine Fmoc-(2-chlorobenzyloxycarbonyl);
3. Lawe ʻia ka huahana me kahi mea hoʻoheheʻe kūpono a hoʻomaʻemaʻe ʻia e ka crystallization e loaʻa ai ka huahana maʻemaʻe.

E pili ana i ka ʻike palekana, Fmoc-(2-chlorobenzyloxycarbonyl) lysine he mea hoʻokalakupua kemika a pono e lawe ʻia nā hana palekana kūpono. Pono e ʻaʻahu ʻia nā mea pale kūpono, e like me nā mīkina lima lab, nā aniani, a me nā lole lab i ka wā o ka hoʻokolohua. Hōʻalo i ka hoʻohu ʻana i ka pauda a i ʻole nā ​​​​mea hoʻonā, pale i ka pili ʻana me ka ʻili a me nā maka. Inā loaʻa ka pilikia, holoi koke me ka wai nui a ʻimi i ke kōkua olakino. E hōʻoia i ka hoʻohana ʻia ʻana i loko o kahi kaiapuni palekana palekana a mālama pono ʻia i mea e pale ai i nā pōʻino.