NN-Bis 9-fluorenylmethyloxycarbonyl-L-histidine CAS 98929-98-7

Ka wehewehe palekana	S22 – Mai hanu i ka lepo. S24/25 – Hōʻalo i ka pili ʻana me ka ʻili a me nā maka.
HS Code	29242990

N(alpha),N(im)-di-fmoc-L-histidine(N(alpha),N(im)-di-fmoc-L-histidine) he pūhui i loaʻa mai ma ka hopena kemika o 9-fluorenecinol a me L -histidine. ʻO kāna kumu kemika C42H38N4O8 a ʻo kona nui molekala pili he 722.78g/mol.N(alpha), ʻo N(im)-di-fmoc-L-histidine kahi hui e pale ai i nā waikawa amino a hoʻohana nui ʻia i ka synthesis organik a me nā hopena biochemical. . Hoʻohana nui ʻia ia e hoʻoponopono i ke ʻano o ka protein a me ka hana, a me ka hoʻonui a hoʻomaikaʻi i ka pae noi o nā kaulahao polypeptide. Hoʻohana nui ʻia kēia pūhui i nā ʻenehana synthesis pae paʻa no ka hoʻololi ʻana i nā koena N-terminus a me histidine o nā peptide a me nā molekole protein.

ʻO ke ʻano o ka hoʻomākaukau ʻana i ka N(alpha),N(im)-di-fmoc-L-histidine maʻamau he ʻekolu mau ʻanuʻu. ʻO ka mua, ua hana ʻia ka ethylene glycol dimethyl ether a me diazotoluene ma lalo o ka catalysis o ka cuprous chloride e hoʻohui i ka 9-fluorenmethanol. A laila, hoʻololi ʻia ka 9-fluorenecinol a me L-histidine ma lalo o nā kūlana acidic e loaʻa ai ka N(alpha),N(im)-di-fmoc-L-histidine. ʻO ka hope, loaʻa ka huahana maʻemaʻe e ka crystallization a me ka hoʻomaʻemaʻe ʻana.

E pili ana i ka ʻike palekana, N(alpha),N(im)-ʻAʻole nui nā hōʻike noiʻi kūpono e pili ana i ka palekana kūikawā o di-fmoc-L-histidine, no laila pono e makaʻala. Ke hoʻohana ʻia i loko o ka hale hana, pono e lawe ʻia nā hana palekana kūpono, me ke komo ʻana i nā mea pale kūpono, e like me nā mīkina lima lima a me nā aniani, a pale aku i ka pili ʻana me ka ʻili a me nā maka. I ka manawa like, pono e mālama ʻia i loko o kahi pahu maloʻo, ventilated a pani ʻia, mai ke ahi a me nā mea puhi. No ka ʻike kikoʻī palekana, makemake ʻia e nīnau i nā palapala kūpono a i ʻole e nīnau i kahi loea.